Demonstration of a Homogeneous Noncompet
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Ryoichi Arai; Hideyuki Nakagawa; Kouhei Tsumoto; Walt Mahoney; Izumi Kumagai; Hi
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Article
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2001
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Elsevier Science
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English
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We describe a noncompetitive homogeneous bioluminescent immunoassay based on the antigen-dependent reassociation of antibody variable domains (open sandwich bioluminescent immunoassay, OS-BLIA). The reassociation of two chimeric proteins, an antibody heavy-chain fragment (V(H))-Renilla luciferase (R