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Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase

โœ Scribed by Yukari Yamakawa; Hiroshi Veda; Atsushi Kitayama; Teruyuki Nagamune


Book ID
114399392
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
852 KB
Volume
93
Category
Article
ISSN
1389-1723

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We describe a noncompetitive homogeneous bioluminescent immunoassay based on the antigen-dependent reassociation of antibody variable domains (open sandwich bioluminescent immunoassay, OS-BLIA). The reassociation of two chimeric proteins, an antibody heavy-chain fragment (V(H))-Renilla luciferase (R