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Homogeneous enzyme-linked competitive binding assay for riboflavin

โœ Scribed by Geun Sig Cha; Mark E. Meyerhoff

Book ID
108304101
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
779 KB
Volume
208
Category
Article
ISSN
0003-2670

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๐Ÿ“œ SIMILAR VOLUMES

Solid phase enzyme-linked competitive bi
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โœ Geun Sig Cha; Mark E. Meyerhoff ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 914 KB

A new solid-phase enzyme-linked assay for riboflavin (vitamin B2) is described. The assay is based on the competition between analyte vitamin molecules and a glucose-6-phosphate dehydrogenase-3-carboxymethylriboflavin conjugate for a limited number of riboflavin-binding protein sites immobilized on

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The use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucosed-phosphate dehydrogenase (G6PDH) and Pgalactosidase (@gal) activities are found which enable the independent measurement of each e