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Dual enzyme labels for simultaneous heterogeneous enzyme-linked competitive binding assays

โœ Scribed by Jeanette M. Buckwalter; Xuan Guo; Mark E. Meyerhoff

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
728 KB
Volume
298
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

The use of two enzyme labels in a dual analyte simultaneous heterogeneous enzyme-linked competitive binding assay is examined. Reaction conditions for monitoring glucosed-phosphate dehydrogenase (G6PDH) and Pgalactosidase (@gal) activities are found which enable the independent measurement of each enzyme activity in the presence of the other. The two enzymes are used as labels in the development of a model simultaneous heterogeneous competitive binding assay for detecting the biotin and vitamin B,, content of vitamin tablets. The simultaneous assay is shown to exhibit analytical dose-response characteristics comparable to those of the single analyte assays. The potential use of the G6PDHIpgal enzyme pair for devising other dual analyte competitive and non-competitive binding assays is discussed.

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