HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas
โ Scribed by OHASHI, H; HASHIZUME, S; MURAKAMI, H; AIHARA, K; SHINOHARA, K; OMURA, H
- Book ID
- 122302543
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 345 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0309-1651
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## An immunoglobulin (Ig) production stimulating factor (IPSF) for hybridomas was found in spent medium of the human B lymphoblastoid cell line, HO-323. The IPSF was purified by serial use of DEAE chromatography, ultrafilnation, gel filtration and HPLC-DEAE chromatography. Purified IPSF was estim
The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TGx.P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC'/29.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocy