A new parental cell line for human x human hybridoma production

✍ Scribed by S. A. Clark; S. E. James; M. Fitchett

• Book ID: 104741556
• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 605 KB
• Volume: 1
• Category: Article
• ISSN: 0920-9069
• DOI: 10.1007/bf00365082

✦ Synopsis

The potential of a new HAT-sensitive human lymphoblastoid cell line TK6 TGx.P1. as a fusion partner was assessed, by comparison with the established human parental cell line UC'/29.6. Both of these cell lines were fused with the peripheral blood mononuclear cells of a patient with B-chronic lymphocytic leukaemia. The hybridomas generated in these fusion experiments were analysed by the fluorescence activated cell sorter and karyotyping. An anti-idiotype ELISA assay detected the presence of the patient's characteristic idiotype bearing immunoglobulin in the supematant of a number of the hybridoma cell lines generated in both fusions.