HN(α/β-COCA-J) Experiment for Measurement of 1JC′Cα Couplings from Two-Dimensional [15N, 1H] Correlation Spectrum

✍ Scribed by Perttu Permi; Tia Sorsa; Ilkka Kilpeläinen; Arto Annila

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 146 KB
• Volume: 141
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1886

✦ Synopsis

A new method for measurement of one-bond 13 C-13 C ␣ scalar and dipolar couplings from a two-dimensional [ 15 N, 1 H] correlation spectrum is presented. The experiment is based on multiplequantum coherence, which is created between nitrogen and carbonyl carbon for simultaneous evolution of 15 N chemical shift and coupling between 13 C and 13 C ␣ . Optional subspectral editing is provided by the spin-state-selective filters. The residual dipolar dipolar contribution to the 13 C-13 C ␣ coupling can be measured from these simplified [ 15 N, 1 H]-HSQC-like spectra. In this way, without explicit knowledge of carbon assignments, conformational changes of proteins dissolved in dilute liquid crystals can be probed conveniently, e.g., in structure activity relationship by NMR studies. The method is demonstrated with human cardiac troponin C.