An improved J-multiplied method for the measurement of 3J(HN, Hα) from the two-dimensional 15N–1H correlation spectrum
✍ Scribed by Perttu Permi; Ilkka Kilpeläinen; Sami Heikkinen
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 142 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
An improved method utilizing J-multiplication for the measurement of 3 J H N , H ˛ coupling constant is introduced. The current method benefits from slower relaxation rate of heteronuclear multiple-quantum coherence. Furthermore, it combines incrementation periods for J-multiplication and 15 N chemical-shift evolution. This reduces the length of the overall pulse sequence by t 1 . This, together with favourable relaxation properties of multiple-quantum coherence, leads to narrower linewidths in the F 1 dimension relative to the previously presented sensitivity-enhanced, J-multiplied HSQC experiment. The method is demonstrated on human ubiquitin.