Determination of HN,Hαand HN,C′ coupling
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R. Weisemann; H. Rüterjans; H. Schwalbe; J. Schleucher; W. Bermel; C. Griesinger
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Article
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1994
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Springer Netherlands
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English
⚖ 603 KB
Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method