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(H)N(COCA)NH and HN(COCA)NH experiments for 1H-15N backbone assignments in 13C/15N-labeled proteins

✍ Scribed by Clay Bracken; Arthur G. Palmer III; John Cavanagh


Book ID
110374041
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
130 KB
Volume
9
Category
Article
ISSN
0925-2738

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Determination of HN,Hαand HN,C′ coupling
✍ R. Weisemann; H. Rüterjans; H. Schwalbe; J. Schleucher; W. Bermel; C. Griesinger 📂 Article 📅 1994 🏛 Springer Netherlands 🌐 English ⚖ 603 KB

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method