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HIV-1 Protease: Characterization of a Catalytically Competent Enzyme−Substrate Intermediate

✍ Scribed by Porter, David J. T.; Hanlon, Mary H.; Furfine, Eric S.


Book ID
127377495
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
77 KB
Volume
41
Category
Article
ISSN
0006-2960

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HIV-1 Protease Specificity Derived from
✍ D.B. Kassel; M.D. Green; R.S. Wehbie; R. Swanstrom; J. Berman 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 670 KB

A rapid and semiquantitative method is described for determining the relative \(k_{\mathrm{ca}} / K_{m}\) for individual peptides in defined substrate mixtures. The method utilizes electrospray ionization/mass spectrometry alone to semiquantitatively determine relative peptide substrate turnover rat