Hinge region of human IgG2 protein: Conformational studies with monoclonal antibodies
โ Scribed by Harada Shigenori; Takagi Shin; Kosada Yumi; Kondo Eiji
- Book ID
- 118384501
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 630 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0161-5890
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Materials and methods ### 2.1 Immunological reagents and buffers Anti-C1 q was supplied by Dako (Copenhagen, Denmark). All other antibodies and conjugates were made in our laboratory. Isotonic Verona1 buffer of pH 7.3 was prepared with 1 mM MgCI2 and 1 mM CaCI2.
Hybrid cell lines which secreted antibodies to liver-specific membrane lipoprotein (LSP) were obtained by immunizing SMA and BALB/c mice with human LSP and fusing their splenocytes with the myeloma cell line P3-NSI/1-Ag4-1. The secretion of antibody to LSP (anti-LSP) was monitored by binding to a hu