Highly sensitive high-performance liquid chromatography for the measurement of malondialdehyde in biological samples
β Scribed by Kenji Fukunaga; Tetsuya Suzuki; Kozo Takama
- Book ID
- 103804464
- Publisher
- Elsevier Science
- Year
- 1993
- Weight
- 401 KB
- Volume
- 621
- Category
- Article
- ISSN
- 0378-4347
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π SIMILAR VOLUMES
A simple, rapid, highly sensitive and reproducible quantification method for plasma malondialdehyde (MDA) was developed using high-performance liquid chromatography (HPLC). The present method is based on the thiobarbituric acid (TBA) reaction and reversed-phase HPLC separation with fluorescence dete
Malondialdehyde, which is generated by lipid peroxidation, can form DNA-protein and/or interstrand DNA crosslinks. The biological consequences of inaccurate repair of these crosslinks may be severe. The expected levels of crosslinking of DNA in vivo are low, and an extremely sensitive method must be