Highly purified detergent-solubilized NADPH-cytochrome P-450 reductase from phenobarbital-induced rat liver microsomes
โ Scribed by Janice L. Vermilion; Minor J. Coon
- Book ID
- 118309284
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 549 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
NADPH:cytochrome c (cytochrome P-450) reductase (Fp) from hamster liver microsomes has been purified to near homogeneity using a simple and rapid method. Microsomes were treated with the detergent Chaps (3-[(3-cholamidopropyl)dimethylammonio]propanesulfonic acid) in combination with 0.07% protamine
NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimm