Coupling of peptides to protein carriers
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G.P. Samokhin; I.N. Filimonov
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Article
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1985
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Elsevier Science
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English
โ 346 KB
Carboxyl groups of succinylated bovine serum albumin were activated by isobutylchloroformate in dimethylformamide solution. Subsequent reaction of the mixed anhydride with amino groups of the added peptide provided rapid and efficient coupling of peptide to protein. For different peptides the yield