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High-Voltage Electroporation of Bacteria: Genetic Transformation of Campylobacter jejuni with Plasmid DNA

✍ Scribed by Jeff F. Miller, William J. Dower and Lucy S. Tompkins

Book ID
123635385
Publisher
National Academy of Sciences
Year
1988
Tongue
English
Weight
853 KB
Volume
85
Category
Article
ISSN
0027-8424

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Transformation of bacteria with plasmid
Transformation of bacteria with plasmid DNA by electroporation
✍ Stefan Fiedler; Reinhard Wirth πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 565 KB

The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (Enterococcus faecalis) and two gram-negative bacteria (Escherichia coli and Pseudomonas putida) with plasmid DNA was investigated. E. faecalis protoplasts could be transformed by electroporati