๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

High voltage electrophoresis of amino acids in urine containing ampicillin

โœ Scribed by Le Phuc Thuy; William L. Nyhan

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
431 KB
Volume
26
Category
Article
ISSN
0009-9120

No coin nor oath required. For personal study only.

โœฆ Synopsis

Analysis of amino acids is complicated by treatment with ampicillin. High voltage electrophoresis, which is convenient for the'qualitative assessment of metabolic diseases, yields smears of amoicillin that mask the bands of citrulline, homocitrulline, phenyiaianine, cystine, and homocystine. The addition of penicillinase prior to high voltage electrophoresis eliminates ampicillin and other penicillins and reveals these key amino acids.

๐Ÿ“œ SIMILAR VOLUMES

Assay of free tryptophan in urine by hig
Assay of free tryptophan in urine by high-voltage electrophoresis
โœ Raul A. Wapnir; Samuel P. Bessman ๐Ÿ“‚ Article ๐Ÿ“… 1965 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 453 KB

With the exception of a calorimetric method (1)) now known to give excessively high results, the assay of tryptophan in urine has been possible only by microbiological methods. At,tempts to apply the spectrofluorometric method of Duggan and Udenfriend (2) directly to urine or to partially partitione

Separation and determination of cross-li
Separation and determination of cross-linking amino acids of elastin by high-voltage paper electrophoresis
โœ E. Moczar; B. Robert; L. Robert ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 730 KB

## Purification of elastin can be accomplished by hydrolytic procedures using acids or alkali, enzymes, or autoclaving (l-3). The resulting preparations can be standardized and characterized only by their amino acid composition (1,4,5) and mainly by their desmosine and isodesmosine contents (6-9)

New procedure of high-voltage electropho
New procedure of high-voltage electrophoresis in polyacrylamide gel and its application to the sequencing of nucleic acids
โœ T.V. Kutateladze; V.D. Axelrod; V.G. Gorbulev; S.N. Belzhelarskaya; R.M. Vartiky ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 586 KB

## Fractionation of nucleic acids and their fragments with polyacrylamide gel has been widely applied in sequencing of nucleic acids. Although the conditions of electrophoresis for this purpose have previously been suggested, we have found that polyacrylamide gel electrophoresis at 5000 V (100 V/c