The chromato\_mphic separations of peptides and proteins on commercially available NO-and 300~ii pore size reversed-phase columns have been compared using various buffer systems. The larger-pore-size packing exhibits a slightly more hydrophilic character while maintaining flow and back-pressure char
✦ LIBER ✦
High velocity reversed-phase chromatography of proteins and peptides: use of conventional C18, 300 Å, 15 μm particles
✍ Scribed by William Kopaciewicz; Elizabeth Kellard; Geofrrey B. Cox
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 799 KB
- Volume
- 690
- Category
- Article
- ISSN
- 1873-3778
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Following previous studies of the use of non-porous monodisperse 1.5-pm noctyl-and n-octadecyl-bonded silicas in gradient elution of proteins, this work was aimed at elucidating further the properties of this novel column material for peptide and protein separations in comparison with wide-pore sili