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Comparison of the high-performance liquid chromatography of peptides and proteins on 100- and 300-Å reversed-phase supports

✍ Scribed by Kenneth J Wilson; Erika Van Wieringen; Stefan Klauser; Martin W Berchtold; Graham J Hughes

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 698 KB
Volume: 237
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)97627-1

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✦ Synopsis

The chromato_mphic separations of peptides and proteins on commercially available NO-and 300~ii pore size reversed-phase columns have been compared using various buffer systems. The larger-pore-size packing exhibits a slightly more hydrophilic character while maintaining flow and back-pressure characteristics typical of 10-p supports. In addition to equal or improved resolving capabilities for smaller amino acid derivatives and peptides, this column material is notably superior to the lCKl-A supports for the chromatography of proteins with molecular weights exceeding ca. 15,OCKl.

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