𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-throughput screening of activity and enantioselectivity of esterases

✍ Scribed by Böttcher, Dominique; Bornscheuer, Uwe T

Book ID
109945978
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
204 KB
Volume
1
Category
Article
ISSN
1750-2799

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

High-throughput screening of activity an
High-throughput screening of activity and enantioselectivity of esterases
✍ Böttcher, Dominique; Bornscheuer, Uwe T 📂 Article 📅 2006 🏛 Nature Publishing Group 🌐 English ⚖ 204 KB

A procedure for the high-throughput screening of esterases is described. This includes enzyme expression in microtiter plates and the measurement of activity and enantioselectivity (E) of the esterase variants using acetates of secondary alcohols as model substrates. Acetic acid released is converte