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High-Throughput Screening Assay for Modulators of Tubulin Tyrosine Ligase

✍ Scribed by Mukherjee, Kamalika; Kong, Lance S.; Dilek, Ozlem; Yoffe, Paul G.; Bane, Susan L.


Book ID
122230874
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
36 KB
Volume
104
Category
Article
ISSN
0006-3495

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In Vitro High-Throughput Screening Assay
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We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur