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A High-Throughput Assay for Modulators of Ligand-Gated Chloride Channels

✍ Scribed by Hamelin, Michel; Meng, Xin; Cuddy, Michael; Korsun, Keith; Ondeyka, John; Simpson, Peter B.; Cully, Doris F.; Priest, Birgit T.

Book ID
121414295
Publisher
Mary Ann Liebert
Year
2005
Tongue
English
Weight
115 KB
Volume
3
Category
Article
ISSN
1540-658X

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✍ LeΓ³n F. Garcı́a-Martı́nez; Graham K. Bilter; Jun Wu; Joe O'Neill; Miguel S. Barb πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 459 KB

We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur