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High-Throughput Scintillation Proximity Assay for Stearoyl-CoA Desaturase-1

✍ Scribed by Tawa, P. ;Falgueyret, J.-P. ;Guiral, S. ;Isabel, E. ;Powell, D. A. ;Zuck, P. ;Skorey, K.


Book ID
111694935
Publisher
SAGE Publications
Year
2011
Tongue
English
Weight
706 KB
Volume
16
Category
Article
ISSN
2472-5552

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An ATPase Assay Using Scintillation Prox
✍ Jamie A. Jeffery; Jeffrey R. Sharom; Monika Fazekas; Penny Rudd; Ewald Welchner; πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 205 KB

A new procedure for measuring ATPase activity in which β₯-33 P-labeled inorganic orthophoshate is de- tected by addition of ammonium molybdate followed by selective adsorption of the resulting phosphomolybdate to scintillation proximity beads in the presence of cesium chloride is described. This meth