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A High Throughput Scintillation Proximity Imaging Assay for Protein Methyltransferases

✍ Scribed by Ibanez, Glorymar; Shum, David; Blum, Gil; Bhinder, Bhavneet; Radu, Constantin; Antczak, Christophe; Luo, Minkui; Djaballah, Hakim


Book ID
117997531
Publisher
Bentham Science Publishers
Year
2012
Tongue
English
Weight
629 KB
Volume
15
Category
Article
ISSN
1386-2073

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An ATPase Assay Using Scintillation Prox
✍ Jamie A. Jeffery; Jeffrey R. Sharom; Monika Fazekas; Penny Rudd; Ewald Welchner; πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 205 KB

A new procedure for measuring ATPase activity in which β₯-33 P-labeled inorganic orthophoshate is de- tected by addition of ammonium molybdate followed by selective adsorption of the resulting phosphomolybdate to scintillation proximity beads in the presence of cesium chloride is described. This meth