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High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery

✍ Scribed by Dr. Tae-Wan Kim; Dr. Harshal A. Chokhawala; Dr. Matthias Hess; Craig M. Dana; Zachary Baer; Dr. Alexander Sczyrba; Prof. Edward M. Rubin; Prof. Harvey W. Blanch; Prof. Douglas S. Clark

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
573 KB
Volume
50
Category
Article
ISSN
0044-8249

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In Vitro High-Throughput Screening Assay
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✍ LeΓ³n F. Garcı́a-Martı́nez; Graham K. Bilter; Jun Wu; Joe O'Neill; Miguel S. Barb πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 459 KB

We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur