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High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplification

✍ Scribed by Fawad A Faruqi; Seiyu Hosono; Mark D Driscoll; Frank B Dean; Osama Alsmadi; Rajanikanta Bandaru; Gyanendra Kumar; Brian Grimwade; Qiuling Zong; Zhenyu Sun; Yuefen Du; Stephen Kingsmore; Tim Knott; Roger S Lasken


Book ID
115003608
Publisher
BioMed Central
Year
2001
Tongue
English
Weight
891 KB
Volume
2
Category
Article
ISSN
1471-2164

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Background: Genetic diversity can help explain disease susceptibility and differential drug response. The most common type of variant is the single nucleotide polymorphism (SNP). We present a low-cost, high throughput assay for SNP genotyping. Methods: The assay uses oligonucleotide probes covalentl