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High-throughput cell-free systems for synthesis of functionally active proteins

✍ Scribed by Alexander S. Spirin


Book ID
113931811
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
340 KB
Volume
22
Category
Article
ISSN
0167-7799

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The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the luciferase gene, in vitro translation of M-hirudin is highly robust against changes in concentrations of K Ψ‰ (and Rb Ψ‰ ). In addition, M-hirud