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A bilayer cell-free protein synthesis system for high-throughput screening of gene products

✍ Scribed by Tatsuya Sawasaki; Yoshinori Hasegawa; Masateru Tsuchimochi; Nami Kamura; Tomio Ogasawara; Toshihiro Kuroita; Yaeta Endo


Book ID
117103943
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
221 KB
Volume
514
Category
Article
ISSN
0014-5793

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✍ RenΓ© Hempel; Frank Wirsching; Andreas Schober; Andreas Schwienhorst πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 147 KB

The properties of M-hirudin as a new reporter gene system were examined using rabbit reticulocyte lysate for cell-free protein expression. In contrast to the luciferase gene, in vitro translation of M-hirudin is highly robust against changes in concentrations of K Ψ‰ (and Rb Ψ‰ ). In addition, M-hirud