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High throughput assay for inhibitors of the epidermal growth factor receptor-associated tyrosine kinase

โœ Scribed by Ivan C. King; Ming Feng; Joseph J. Catino


Book ID
118346374
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
475 KB
Volume
53
Category
Article
ISSN
0024-3205

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Activation of cells is frequently followed by tyrosine phosphorylation of proteins. To quantify this process, we developed a ratiometric enzyme-linked immunosorbent assay (ELISA) using epidermal growth factor receptors (EGFR) as a model. Microtiter dishes were coated with anti-EGFR monoclonal antibo