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High-resolution tomographic diffractive microscopy of biological samples

✍ Scribed by Bertrand Simon; Matthieu Debailleul; Anne Beghin; Yves Tourneur; Olivier Haeberlé

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
264 KB
Volume
3
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolution. The authors now study its use for biological applications, and imaged several preparations with fluorescence confocal microscopy and tomographic diffractive microscopy. The results highlight some interesting features of this instrument, which should attract the interest of biologists for this new technique. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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