𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-resolution nmr study of yeast tRNA and the native and denatured conformers of yeast tRNA

✍ Scribed by B. F. Rordorf; D. R. Kearns; Erin Hawkins; S. H. Chang

Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
609 KB
Volume
15
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The high‐resolution nmr spectrum of baker's yeast tRNA, a recently sequenced non‐denaturable tRNA, has been compared with the spectra of the native and denatured conformers of the closely related species tRNA. Because of the presence of many common base pairs in the different tRNA's, it is possible to assign most of the low‐field resonances to specific secondary‐structure base pairs. A comparison of the observed positions of the various resonances with those predicted by a semiempirical ring‐current shift theory shows a root‐mean‐square deviation of 0.14, 0.11, and 0.12 ppm for tRNA (native), and tRNA (denatured), respectively. These results support the ring‐current shift theory currently used to interpret the low‐field nmr spectra of the tRNA molecules. Differences between the predicted and observed positions of some resonances provide new evidence for higher order effects such as shifts from second nearest neighbors, anomalous shifts exerted by G·U base pairs, and tertiary‐structure effects. A model that was previously proposed for the denatured conformer of tRNA is also supported.

📜 SIMILAR VOLUMES

Reintroduction of a characterized Mit tR
Reintroduction of a characterized Mit tRNA glycine mutation into yeast mitochondria provides a new tool for the study of human neurodegenerative diseases
✍ H. Rohou; S. Francisci; T. Rinaldi; L. Frontali; M. Bolotin-Fukuhara 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 220 KB 👁 3 views

We report the identification and characterization of a new mutation (ts9) in the Saccharomyces cerevisiae mitochondrial genome, which was first genetically mapped in the tRNAgly region and further identified by means of sequencing as consisting of a G to A transition at position 30 in the tRNA. The

The Sequence of a 54·7 kb Fragment of Ye
The Sequence of a 54·7 kb Fragment of Yeast Chromosome XV Reveals the Presence of Two tRNAs and 24 New Open Reading Frames
✍ VALENS, MICHELE; BOHN, CHANTAL; DAIGNAN-FORNIER, BERTRAND; DANG, VAN-DINH; BOLOT 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 307 KB 👁 2 views

A 54 719 bp fragment from the right arm of Saccharomyces cerevisiae chromosome XV has been sequenced from the inserts of two cosmids (pEOA213 and pEOA217). The computer analysis of this sequence has revealed the presence of eight known genes (CKA2, CYC1, ALG8, TCM1, TMP1, UFE1, RTS2 and ASE1) and fo

The Nucleotide Sequence of a 39 kb Segme
The Nucleotide Sequence of a 39 kb Segment of Yeast Chromosome IV: 12 New Open Reading Frames, Nine Known Genes and One Gene for Gly-tRNA
✍ BAHR, ANDRÉ; MÖLLER-RIEKER, SABINE; HANKELN, THOMAS; KRAEMER, CHRISTIANE; PROTIN 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB 👁 2 views

The complete nucleotide sequence of a 39 090 bp segment from the left arm of yeast chromosome IV was determined. Twenty-one open reading frames (ORFs) longer than 100 amino acids and a Gly-tRNA gene were discovered. Nine of the 21 ORFs (D0892, D1022, D1037, D1045, D1057, D1204, D1209, D1214, D1219)

Interaction of the antimalarial drug flu
Interaction of the antimalarial drug fluoroquine with DNA, tRNA, and poly(A): 19F-NMR chemical-shift and relaxation, optical absorption, and fluorescence studies
✍ Philip H. Bolton; Peter A. Mirau; Richard H. Shafer; Thomas L. James 📂 Article 📅 1981 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 779 KB

## Abstract The interaction of the fluorinated antimalarial drug fluoroquine [7‐fluoro‐4‐(diethyl‐amino‐1‐methylbutylamino)quinoline] with DNA, tRNA, and poly(A) has been investigated by optical absorption, fluorescence, and ^19^F‐nmr chemical‐shift and relaxation methods. Optical absorption and fl

Study of the behaviour of amino acids in
Study of the behaviour of amino acids in aqueous solution by time-domain NMR and high-resolution NMR
✍ M. Khallouk; D. N. Rutledge; A. M. S. Silva; I. Delgadillo 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 189 KB

## Abstract The study of protein hydration by time‐domain NMR is complicated by the great number of interactions involved, resulting from the presence of several amino acids and the possible modifications produced by the various structures. Moreover, a good comprehension of the molecular interactio