High-resolution mass-spectrometry analysis of peptides and proteins
โ Scribed by Nikolaev, Eugene N; Popov, I A; Kononikhin, A S; Indeykina, M I; Kukaev, E N
- Book ID
- 120018475
- Publisher
- Royal Society of Chemistry
- Year
- 2012
- Tongue
- English
- Weight
- 399 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0036-021X
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A "micro-electrospray" ionization source has been developed that markedly increases the sensitivity of the conventional electrospray source. This was achieved by optimization of the source to accommodate nanoliter flow rates from 300 to 800-nL/min spraying directly from a capillary needle that, for
## Abbreviations CE capillary electrophoresis CID collision-induced dissociation EGF epidermal growth factor ESI electrospray ionization HPLC high-performance liquid chromatography ICP inductively coupled plasma IMAC immobilized metal affinity chromatography MALDI matrix-assisted laser desorption/