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High-resolution mapping of a minor histocompatibility antigen gene on mouse chromosome 2

โœ Scribed by Aamir R. Zuberi; Derry C. Roopenian

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
779 KB
Volume
4
Category
Article
ISSN
0938-8990

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โœฆ Synopsis

Minor histocompatibility (H) loci are significant tissue transplantation barriers but are poorly understood at the genetic and molecular level. We describe the construction of a high-resolution genetic map that positions a class II MHC-restricted minor H antigen locus and orders 12 other genes and genetic markers within the we-un interval of mouse Chromosome (Chr) 2. An intersubspecific backcross between BIO.UW/Sn-H-3 v and CAST/El, an inbred stock of Mus musculus castaneus, was used for this purpose. A total of 1168 backcross mice were generated, and 71 we-un recombinants were identified. Significant compression of the genetic map in males versus females and transmission distortion of CAST-derived we, un, and A w genes were observed. Monoclonal T cell lines specific for two minor H alloantigens, Hd-1 a and Hd-2 a, encoded by gene(s) that map to the we-un interval were used to antigen type the backcross mice. The results suggest the Hd-1 a and Hd-2 a antigens are most likely encoded by a single gene, now referred to as H-3b. The determined gene order is we-O.09 +-0.09-

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