High-Resolution Density Gradient Sedimentation Analysis
β Scribed by Britten, R. J.; Roberts, R. B.
- Book ID
- 121165526
- Publisher
- American Association for the Advancement of Science
- Year
- 1960
- Tongue
- English
- Weight
- 410 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0036-8075
No coin nor oath required. For personal study only.
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## High resolution density gradient electrophoresis of cellular organelles A density gradient electrophoresis apparatus made of Perspex was constructed, with a separation column (7 X 2.2 cm) containing a 0-5% linear Ficoll gradient. The useful separation path is 6 cm. A specially designed gradient
Two models of sedimentation in a density gradient are analyzed. The firht is f(ir sedimentation in cylindrical sector geometry and contains the assumption that diff i w i o i i can be neglected. The second treats sedimentation in a rectangular field and includediffusion, although the boundaries are
## High-resolution density gradient electrophoresis of proteins and subcellular organelles Following a concept developed by Bier et al. (Electrophoresis 1993, 14, [1011][1012][1013][1014][1015][1016][1017][1018], binary mixtures of amphoteric buffers with low conductivity and a good buffering capa