High-resolution density-gradient analysis of sea urchin polysomes
β Scribed by Geraldine H. Cohen; R.M. Iverson
- Book ID
- 118850287
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 368 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0006-291X
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Unsheared chromatin isolated from sea urchin embryos was submitted to buoyant density centrifugation in sucrose-glucose gradients. The main peak of blastula chromatin was at a density position of 1.299 plus or minus 0.028 plus or minus 0.009 g ml -minus 1 whereas at gastrula stage a shift to a lower
## High resolution density gradient electrophoresis of cellular organelles A density gradient electrophoresis apparatus made of Perspex was constructed, with a separation column (7 X 2.2 cm) containing a 0-5% linear Ficoll gradient. The useful separation path is 6 cm. A specially designed gradient
## High-resolution density gradient electrophoresis of proteins and subcellular organelles Following a concept developed by Bier et al. (Electrophoresis 1993, 14, [1011][1012][1013][1014][1015][1016][1017][1018], binary mixtures of amphoteric buffers with low conductivity and a good buffering capa