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High resolution chromatography of ribonucleosides and its application to RNA analysis

✍ Scribed by Kouji Tanaka; Kouhei Yazawa; Terumi Nakajima

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 417 KB
Volume: 3
Category: Article
ISSN: 0269-3879
DOI: 10.1002/bmc.1130030604

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and precise method was developed for the separation of nucleosides including modified nucleosides and oligonucleotides. Nineteen kinds of nucleosides were completely separated by HPLC using an ODS column (TSK-gel ODS 80TM) and aqueous mobile phases. The RNA molecule was digested by base restrictive RNase (RNase A, RNase T,) and the digests were separated chromatographically into each oligonucleotide. The nucleoside composition of an oligonucleotide was then determined by this analytical system. It is thus possible to fit the oligonucleotide in the original RNA molecule by using modified bases as markers. The reaction site of quinacrine mustard for tRNAPhe (from yeast) could be determined by this analytical system.

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