High-resolution 1H and 2H NMR spectroscopy of pure essential fatty acids for plants and animals

✍ Scribed by M. Aursand; F. Mabon; G. J. Martin

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 305 KB
• Volume: 35
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/(sici)1097-458x(199712)35:13<s91::aid-omr201>3.0.co;2-g

✦ Synopsis

High-resolution 1H and 2H NMR spectroscopy were used to study the most important saturated (C14 : 0, C16 : 0, C18 : 0), monounsaturated (C16 : 1n-7, C18 : 1n-7, C18 : 1n-9, C20 : 1n-9, C22 : 1n-9) and polyunsaturated [ C18 : 3n-3, C20 : 5n-3 (EPA), C22 : 6n-3 (DHA) ] fatty acids which may be found in Ðsh lipids, in order to prepare the tools for a subsequent study of the recognition of the origin of Ðsh oils by isotopic analysis. The interpretation of the 1H and 2H NMR spectra of the di †erent acids is reported. Additionally, the overall D/H and the 13C/12C isotope ratios are given. The overall D/H ratio ranges from 114.0 to 137.2 ppm/V.SMOW (V.SMOW = water standard) and the 13C/12C ratio from Ô23.5 to From the 2H NMR spectra, an investigation of the internal Ô33.2&. deuterium distribution in the saturated and mono-and polyunsaturated fatty acids was made. The deuterium distribution can be given for nearly all the chemical sites, except for the di †erent double bonds, in EPA and DHA. The precision of the measurements was of the order of 0.5-4% depending on the nature of the cluster considered.