High production of Poly-β-hydroxybutyrate (PHB) fromMethylobacterium organophilumunder potassium limitation

✍ Scribed by Seon Won Kim; Pil Kim; Hyun S. Lee; Jung H. Kim

• Book ID: 104637178
• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 415 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00137805

✦ Synopsis

Mass production

of Poly@-hydroxybutyrate) (PHB) from Mrthylobtrcteriunl organophilum under potassium-limited condition was carried out using a microcomputeraided automatic fed-batch culture system. The concentration of methanol was kept within the range of 2 -3 g/l which did not show any inhibitory effect on cell growth. The PHB accumulation was stimulated when potassium concentration in the culture broth fell below 25 mg/l. After 70 hours of cultivation, the concentrations of cell mass and PHB were obtained to be 250 g/l and 130 g/l, respectively, which corresponded to a volumetric productivity of 1.8 -2.0 g-PHB/l-hr. PHB contents ranged from 52 '% to 56 % of dry cell weight with a yield factor (Yws) of 0.19 g-PHB/g-methanol.