✦ LIBER ✦

High-pressure liquid chromatography: A tool for study of enzyme reactions involving purines and purine nucleotides

✍ Scribed by Barbara Vasquez; Allan L. Bieber

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 325 KB
Volume: 79
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90377-3

No coin nor oath required. For personal study only.

✦ Synopsis

High-pressure liquid chromatography (HPLC) has been used as a tool to assay IMP-GMP:pyrophosphate phosphoribosyltransferase (HGPRT). Complete separation of the products of the enzyme-catalyzed reactions from the substrates and high sensitivity obviate some of the problems encountered when spectral assays are used. Results of divalent metal ion studies with HGPRT demonstrate the utility of the assay method. 52

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