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High-pressure liquid chromatographic analysis of phenylpropanolamine in human plasma following derivatization with O-phthalaldehyde

✍ Scribed by William D. Mason; Edwin N. Amick

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
356 KB
Volume
70
Category
Article
ISSN
0022-3549

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✦ Synopsis

A high-pressure liquid chromatographic analysis of phenylpropanolamine in human plasma following extraction, back-extraction, and 0-phthalaldehyde derivatization is presented. Using fluorescence detection, the method was sufficiently sensitive to quantitate as little as 5 ng of drug/ml of plasma; the coefficient of variation below 100 ng/ml ranged between 5.7 and 2.8%. Plasma concentration data following a single 25-mg dose of phenylpropanolamine hydrochloride in 12 healthy volunteers demonstrate the application of the analytical method. 0 -phthalaldehyde, separation by high-pressure liquid chromatography (HPLC), and detection by fluorescence

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