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High-performance liquid chromatographic analysis of amino acids in physiological fluids: On-line precolumn derivatization with o-phthaldialdehyde

✍ Scribed by Marianne O. Fleury; David V. Ashley

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 453 KB
Volume: 133
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90092-1

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✦ Synopsis

The reaction between primary amines and ephthaldialdehyde in the presence of a thiol was exploited to measure the concentrations of 2 1 amino acids in serum by high-performance liquid chromatography with tluorescent detection. The method described here includes an automatic on-line precolumn procedure for derivatizing the amino acids, permitting ml1 automation and avoiding problems due to time-dependent decay of fluorescence of the derivatives. The total analysis time is less than 60 min and limits of sensitivity are about 100 fmol. Proline, hydroxyproline, and cysteine are not detected. Comparison with data generated by a standard amino acid analyzer shows this technique to be reliable.

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