High physiological thermal triplex stability optimization of twisted intercalating nucleic acids (TINA)
✍ Scribed by Bomholt, Niels; Osman, Amany M. A.; Pedersen, Erik B.
- Book ID
- 118737878
- Publisher
- Royal Society of Chemistry
- Year
- 2008
- Tongue
- English
- Weight
- 241 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1477-0520
- DOI
- 10.1039/B808564A
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## Abstract A highly efficient method for postsynthetic modification of unprotected oligonucleotides incorporating internal insertions of (__R__)‐1‐__O__‐(4‐ethynylbenzyl)glycerol has been developed through the application of click chemistry with water‐insoluble pyren‐1‐yl azide and water‐soluble b
## Abstract A postsynthetic, on‐column Sonogashira reaction was applied on DNA molecules modified by 2‐ or 4‐iodophenylmethylglycerol in the middle of the sequence, to give the corresponding __ortho__‐ and __para__‐twisted intercalating nucleic acids (TINA) with 1‐, 2‐, and 4‐ethynylpyrene residues