A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of
β¦ LIBER β¦
High pH Solubilization and Chromatography-Based Renaturation and Purification of Recombinant Human Granulocyte Colony-Stimulating Factor from Inclusion Bodies
β Scribed by Ming Li; Hua Fan; Jiahua Liu; Minhong Wang; Lili Wang; Chaozhan Wang
- Book ID
- 107566134
- Publisher
- Springer-Verlag
- Year
- 2012
- Tongue
- English
- Weight
- 370 KB
- Volume
- 166
- Category
- Article
- ISSN
- 0273-2289
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The synthesis and secretion of recombinant human granulocyte colony-stimulating factor (rhG-CSF) are investigated in fed-batch cultures at high cell concentration of recombinant Saccharomyces cerevisiae, and some important characteristics of the secreted rhG-CSF are demonstrated. Transcription of th