𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High pH Solubilization and Chromatography-Based Renaturation and Purification of Recombinant Human Granulocyte Colony-Stimulating Factor from Inclusion Bodies

✍ Scribed by Ming Li; Hua Fan; Jiahua Liu; Minhong Wang; Lili Wang; Chaozhan Wang


Book ID
107566134
Publisher
Springer-Verlag
Year
2012
Tongue
English
Weight
370 KB
Volume
166
Category
Article
ISSN
0273-2289

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


High level expression and simple purific
✍ Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-II Park; Se-Yong Lee; Young πŸ“‚ Article πŸ“… 1995 πŸ› Springer Netherlands 🌐 English βš– 345 KB

A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of

Enhanced secretion of human granulocyte
✍ Cheon Soon Bae; Doo Suk Yang; Ki Ryong Chang; Baik Lin Seong; Jeewon Lee πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 265 KB πŸ‘ 2 views

The synthesis and secretion of recombinant human granulocyte colony-stimulating factor (rhG-CSF) are investigated in fed-batch cultures at high cell concentration of recombinant Saccharomyces cerevisiae, and some important characteristics of the secreted rhG-CSF are demonstrated. Transcription of th