High-Performance Membrane Chromatography. A Novel Method of Protein Separation
โ Scribed by Tennikova, T. B.; Svec, F.; Belenkii, B. G.
- Book ID
- 118236833
- Publisher
- Informa UK (Taylor & Francis)
- Year
- 1990
- Tongue
- English
- Weight
- 283 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0148-3919
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Ceramic hydroxyapatite high-performance liquid chromatography with a solvent containing high concentrations of salts was used for the separation of membrane proteins solubilized under nondenaturing and high salt conditions. The chromatographic conditions were optimized using sodium cholate as the de
Plasma membrane proteins from liver were analysed by concanavalin A affinity and immunoaffinity high-performance liquid chromatography. In the method, four peptide bands with apparent molecular weights of 140,000, 120,000, 80,000 and 60,000 could be isolated. In the second method, with two immobiliz