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High-performance liquid chromatography/electrospray ionization tandem mass spectrometry of hydroxylated uroporphyrin and urochlorin derivatives formed by photochemical oxidation of uroporphyrinogen I

✍ Scribed by Malcolm Danton; Chang Kee Lim

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
575 KB
Volume
21
Category
Article
ISSN
0269-3879

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✦ Synopsis

Abstract

Hydroxylated uroporphyrin I and urochlorin I derivatives formed by photochemical oxidation of uroporphyrinogen I were separated by high‐performance liquid chromatography and fully characterized by electrospray ionization tandem mass spectrometry. The porphyrins and chlorins were identified by analysis of their product ion spectra with each hydroxylated derivative giving a characteristic collision‐induced dissociation fragmentation pattern. The porphyrins and chlorins characterized were meso‐hydroxyuroporphyrin I, α‐hydroxypropionic acid uroporphyrin I, β‐hydroxypropionic acid uroporphyrin I, hydroxyacetic acid uroporphyrin I, __trans‐__7‐hydroxy‐8‐spirolactoneurochlorin I, __cis‐__7‐hydroxy‐8‐spirolactoneurochlorin I and trans‐ and __cis‐__7,8‐dihydroxyurochlorins I. Copyright © 2007 John Wiley & Sons, Ltd.

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