Purification of adenovirus hexon protein
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Matti Waris; Pekka Halonen
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Article
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1987
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Elsevier Science
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English
⚖ 459 KB
Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column i