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High-performance liquid chromatography of proteins

✍ Scribed by Shung Ho Chang; Karen M. Gooding; Fred E. Regnier


Book ID
108335694
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
862 KB
Volume
125
Category
Article
ISSN
1873-3778

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The ability of two high-performance liquid chromatography gel permeation columns to separate proteins was evaluated. These columns gave satisfactory molecular weight separations for some, but not all, proteins tested. These results indicate that there are limitations in confidence of molecular weigh

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## Abstract A microchromatographic procedure for highly sensitive analysis of proteins has been developed by using short capillary columns with reverse‐phase, ion‐exchange, and hydrophobic interaction sorbents. The high analytical mass sensitivity on the level of several nanograms was attained by t