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High-performance liquid chromatographic separation and quantitation of benzisoquinolinedione (Nafidimide) from biological fluids

โœ Scribed by Katherine Lu; Nimarol Savaraj; Robert A. Newman

Book ID
107996519
Publisher
Elsevier Science
Year
1985
Weight
340 KB
Volume
345
Category
Article
ISSN
0378-4347

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High-performance liquid chromatographic
High-performance liquid chromatographic separation and quantitation of tetrapyrroles from biological materials
โœ Herbert L. Bonkovsky; Sheryl G. Wood; Scott K. Howell; Peter R. Sinclair; Beth L ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 662 KB

We describe a rapid, reverse-phase HPLC procedure for separating and quantifying tetrapyrroles of biological interest. This procedure uses a 5-micron C18 column and the mobile phase is ammonium phosphate (pH 3.5) with a methanol gradient that is increased from 61 to 100%. Detection is by absorbance