High-performance liquid chromatographic
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Herbert L. Bonkovsky; Sheryl G. Wood; Scott K. Howell; Peter R. Sinclair; Beth L
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Article
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1986
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Elsevier Science
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English
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We describe a rapid, reverse-phase HPLC procedure for separating and quantifying tetrapyrroles of biological interest. This procedure uses a 5-micron C18 column and the mobile phase is ammonium phosphate (pH 3.5) with a methanol gradient that is increased from 61 to 100%. Detection is by absorbance