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High performance liquid chromatographic resolution of the optical isomers of D,L-tryptophane, D,L-5-hydroxytryptophan and D,L-dopa on cellulose columns

✍ Scribed by Gübitz, G. ;Jellenz, W. ;Schönleber, D.

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
152 KB
Volume
3
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

The application of HPLC for the purpose of separation of some amino acid racemates with aromatic substituents is described.

Using simple untreated cellulose with an average particle size of 7 μm as stationary phase, complete resolution of D,L‐tryptophane and D,L‐5‐hydroxytryptophane can be obtained. D,L‐3,4‐dihydroxyphenylalanine is resolved partially.

The α‐value and resolution for D,L‐tryptophane was found to be 1.5.

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