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High-performance liquid chromatographic method for the evaluation of possible interferences in basophil-histamine release measurements

โœ Scribed by Paul D. Siegel; Daniel M. Lewis; Stephen A. Olenchock

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
591 KB
Volume
188
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Basophil activation studies commonly rely on the measurement of histamine following extraction and condensation to o-phthalaldehyde (OPT) as a quantitative measurement of degranulation. Specificity has long been recognized as a problem with this method. We have described a new high-performance liquid chromatographic method that allows for both a qualitative and a quantitative check of the purity of the OPT-histamine adduct. This method was sensitive (limit of detection = 2.55 pmol) and linear over a wide range (5 to 1000 ng/ml).

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