High performance liquid chromatographic determination of metoclopramide in plasma and urine and its application to biopharmaceutical investigations

✍ Scribed by Nieder, M. ;Jaeger, H.

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 488 KB
• Volume: 10
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240101208

✦ Synopsis

Anoptimized HPLC method forthe quantificationof metoclopramide (MCP) in human plasma and urine is described. MCP and internal standard are extracted from alkalinized substrate into diethyl ether and back-extracted into dilute acid. The analytes are separated with a ternary mobile phase at cyanopropyl-silica and detected at 312 nm (UV detection). The lower limit of quantification is 0.5 ng/ml in plasma and 50 n g h l in urine. Optimization of extraction, chromatography, and detection is discussed. The method is selective to numerous common drug substances with excellent accuracy and precision data. After validation, the method is applied to the samples of a pharmacokinetic study. Pharmacokinetic parameters indicate the need for a sophisticated method as tool for optimization of metoclopramide formulations.